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Image Search Results
Journal: Nature neuroscience
Article Title: Kcnn2 blockade reverses learning deficits in the mouse model of Fetal Alcohol Spectrum Disorders
doi: 10.1038/s41593-020-0592-z
Figure Lengend Snippet: ( a ) Design of the lineage-tracing HSE-FLPo;FRT-STOP-RFP (HSE-RFP) reporter system. ( b ) Experimental timeline. ( c , d ) RFP reporter expression was observed in a subset of GFP + electroporated neurons in layer III of the M1 in PAE mice, but not in control mice. ***P = 0.0003 by two-tailed Student’s t -test (n = 10 animals per group). In box plots ( d ), the line within the box indicates the median, and the upper and lower edges of the box represent the 25th and 75th percentiles, respectively. The upper and lower whisker boundaries indicate the 10th and 90th percentiles, respectively, and dots indicate outliers. ( e ) Representative images of sampling intracellular contents from a single cell (time course, top to bottom). Similar results were obtained with n = 4 animals. ( f ) Principal Component Analysis shows highly variable molecular properties of reporter + neurons in PAE mice (red), segregated from the clusters of reporter − neurons in PAE (green) and control (blue) mice, and the cluster of olfactory bulb (OB) neurons (black) [olfactory bulb neurons: n = 4, reporter + neurons (PAE): n = 15, reporter − neurons (PAE): n= 7, reporter − neurons (control): n = 6]. ( g ) Gene counts against the entropy score among reporter + neurons in PAE mice defined by ROKU. Left and right sides of a broken line include the upregulated and downregulated genes in reporter + neurons, respectively. The genes in yellow bars are considered as specifically changed in reporter + neurons. ( h , i ) K-means clustering and heatmaps of 73 upregulated ( h ) and 20 downregulated ( i ) genes in the reporter + neurons defined by ROKU (in the yellow bars in g ). Gene names (from left to right in h and i ) are re-listed in . Red, green and blue colors in the y-axis correspond to each cell in PCA (see f ).
Article Snippet: A
Techniques: Expressing, Control, Two Tailed Test, Whisker Assay, Sampling